Finally,
the PCR You Truly Need

Beyond complexity. Beyond compromise. Welcome to the future of multiplex PCR development, powered by Mila™ AI. Let us make the Best Kit Possible for you.

Good PCR is No longer Enough.

The complexity of multiplex PCR has always forced compromises:

Why settle for “close enough” when your reality demands precision?

With Mila™, we can create PCR kits tailored from the ground up using artificial intelligence, with the exact targets, coverage, and balance you need.

No guesswork. No compromises. Just the right kit, made possible

Introducing Mila

Let us Design the Impossible.

Mila™ is the world’s first AI-powered platform that liberates multiplex PCR development:

With Mila™, we can create PCR kits tailored from the ground up using artificial intelligence, with the exact targets, coverage, and balance you need.

No guesswork. No compromises. Just the right kit, made possible

What Makes Mila™ Unique

The BEST kit, evading THE REAL COMPLEXITIES of Multiplex PCR.

Full Target
Freedom

Detect multiple organisms at once, no compromises, no artificial limits.

Hardware
Flexibility

Compatible with your preferred PCR systems and chemistries.

Validation
Partnerships

From design to final proof, Mila™ is your guide.

Who We Serve

We Walk with the Visionaries.

Mila™ is trusted by industry pioneers and leaders who refuse to settle. Together, we have created PCR kits that define new standards in food safety, diagnostics, and biotechnology.

Products: Crafted
by Mila™,
Perfected as
Ampliora™

When vision meets intelligence, you get Ampliora™, a line of the best tailor-made PCR kits that redefine what’s possible.

Products we have developed with our clients.

Ampliora™2.3 Listeria spp. and L. monocytogenes SPID

Ampliora™ 2.8 Listeria spp. and Salmonella spp. SPID

Ampliora™ 3.5 Salmonella spp. L. monocytogenes and Listeria spp. SPID

Ampliora™ F39 E. coli STEC, E. coli O157:H7and Salmonella spp. SPID

Ampliora™ 8.1 Beer Yeast Plus SPID

Ampliora™ 8.2 Beer Bacteria Plus SPID

Ampliora™ 4.7 Spoilage Beverage SPID

Ampliora™ 6.1 WaterScan Plus SPID

Why it works

Behind Mila™ lies deep learning, system modeling, and advanced combinatorial logic, all designed to eliminate guesswork and maximize assay performance.

There are more ways to build a 4-target PCR test, than stars in the milky way.

How it works

1

Sequence correction

Align all published sequences for each target. Screen for errors and artifacts.

2

Primer and probe selection

Align all published sequences for each target. Screen for errors and artifacts.

3

Reaction optimization

Thermodynamic modeling and optimization by Al to select ideal reaction conditions and the best possible primer and probe set without testing every possibility.

Say hello to your new microbiology expert

By using our kits, you could access the TXA software for free*. TAAG Xpert Assistant (TxA) is a data-driven and artificial intelligent platform that interacts and analyzes all steps in the microbial food safety chain to help you to maximize the food safety of your plant.
Some features of TxA:

Discover how a Fortune 100 company validated our pathogen PCR kits

You Were Never Meant to Settle.

It’s time to build the PCR kits your vision demands, faster,
smarter, without limits. Let Mila™ be your guide.

Let´s coordinate a meeting to find the best solution for your needs.

Feel free to contact us.

Contact us

A typical qPCR assay requires three oligonucleotides (“oligos”): one forward primer, one reverse primer, and one hydrolysis probe for each target. Each of these oligos must be selected from many possibilities. Furthermore, the best primer/probe set for one target may no longer be the best option once a second target is added, since the second set of oligos can interact with the first. In a modest 4-target qPCR reaction, depicted in the figure above, there may be more possible primer/probe combinations than there are stars in the milky way galaxy. Finding the best combination by trial-and-error is nearly impossible.

 

Mila is a comprehensive platform for in silico PCR design. It retrieves and aligns all published sequences for the targets of interest, distinguishing sequencing errors from true variants and removing them. BLAST algorithms are used to select primers that effectively exclude potential off-target hybridizations while ensuring inclusivity. The remaining primer/probe sets undergo extensive thermodynamic modelling and AI-based optimization to select the set with the highest efficiency and lowest probability of undesirable interactions. This is accomplished in a computationally efficient manner, without testing every possibility. The algorithm considers a comprehensive range of variables, including the thermodynamics of nucleic acids, differences between fluorophores, melting and annealing temperatures, secondary structures, and the chemical properties of the reaction (pH, salt concentrations, other reagents, etc.)

 

This site is registered on wpml.org as a development site. Switch to a production site key to remove this banner.